Site-directed spin labeling of proteins for distance measurements in vitro and in cells.
نویسندگان
چکیده
Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.
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عنوان ژورنال:
- Organic & biomolecular chemistry
دوره 14 24 شماره
صفحات -
تاریخ انتشار 2016